TY  - JOUR
PY  - 2019//
TI  - Upconversion nanoprobes for in vitro and ex vivo measurement of carbon monoxide
JO  - ACS applied materials and interfaces
A1  - Wang, Ningning
A1  - Li, Zuhao
A1  - Liu, Wei
A1  - Deng, Ting
A1  - Yang, Jinfeng
A1  - Yang, Ronghua
A1  - Li, Jishan
SP  - 26684
EP  - 26689
VL  - 11
IS  - 30
N2  - Here, we have developed a new colorimetric and luminescence nanosensor, based on upconversion nanoparticles (UCNP), for in vitro and ex vivo measurement of carbon monoxide (CO). The nanoprobe has two strong fluorescence emission peaks in the UCNP core to excite fluorophore at 540 and 800 nm. The CO-responsive palladium ion-bounded rhodamine B derivatives (Pd-RBDs) is encapsulated in the mesoporous silica (mSiO2) shell and the particles outside the cyclodextrin (CD) layer. Reduction of palladium ions by CO results in the release of palladium from the Pd-RBDs, thereby inducing the closure of the spiro ring of the RBD and the accompanying reduction of rhodamine B (RB) absorption at 500-600 nm overlapping with the luminescence spectrum of UCNPs maximized at 540 nm. Therefore, the I540/I800 ratio of the nanoprobe will increase when CO is present, making it possible to quantitatively measure CO. Besides working in a clean buffer environment with known [CO], this method was evaluated using living cells and tissue sections. Additionally, these probes were also successfully used to investigate the CO-related protective activity of anti-hepatic ischemia-reperfusion injury (HIRI) oligopeptides.<p />  <p>Language: en</p>
LA  - en
SN  - 1944-8244
UR  - http://dx.doi.org/10.1021/acsami.9b08549
ID  - ref1
ER  -