TY - JOUR PY - 2019// TI - Combined DNA typing and protein identification from unfired brass cartridges,, JO - Journal of forensic sciences A1 - Sterling, Stacey-Ann A1 - Mason, Katelyn E. A1 - Anex, Deon S. A1 - Parker, Glendon J. A1 - Hart, Bradley A1 - Prinz, Mechthild SP - ePub EP - ePub VL - ePub IS - ePub N2 - Biological evidence analysis from contact traces is adversely affected by low quantity and quality of DNA. Proteins in these samples contain potentially individualizing information and may be particularly important for difficult surfaces such as brass, where DNA may yield incomplete profiles. In this study, touched unfired brass cartridges were sampled using dry tape or wet swabs and analyzed by separating DNA and protein from the same collected material, thus producing both genomic and proteomic information. DNA recovery was similar for both collection methods, with tape yielding an average of 1.36 ± 1.87 ng and swabs, 1.34 ± 3.04 ng. Analysis by mass spectrometry identified 95 proteins, with the two collection methods showing no significant difference (p = 0.76) in the average number of collected proteins: 44.5 ± 10.9, (tape) versus 47.9 ± 20.4 (swabs). Proteins can be collected from fingerprints at levels necessary to provide identifying information, thus expanding information obtained from challenging evidence.

© 2019 American Academy of Forensic Sciences.

Language: en

LA - en SN - 0022-1198 UR - http://dx.doi.org/10.1111/1556-4029.14042 ID - ref1 ER -