TY  - JOUR
PY  - 2018//
TI  - The protection effect and mechanism of hyperbaric oxygen therapy in rat brain with traumatic injury
JO  - Acta cirúrgica Brasileira
A1  - Xing, Pengcheng
A1  - Ma, Ke
A1  - Li, Lijuan
A1  - Wang, Donglian
A1  - Hu, Guoyong
A1  - Long, Wei
SP  - 341
EP  - 353
VL  - 33
IS  - 4
N2  - PURPOSE: To investigate the effect of hyperbaric oxygen therapy (HBOT) on traumatic brain injury (TBI) outcome. <br><br>METHODS: The modified Marmarou's weight drop device was used to generate non-lethal moderate TBI rat model, and further developed in vitro astrocytes culturing system. Then, we analyzed the expression changes of interested genes and protein by quantitative PCR and western blot. <br><br>RESULTS: Multiple HBO treatments significantly reduced the expression of apoptosis promoting genes, such as c-fos, c-jun, Bax and weakened the activation of Caspase-3 in model rats. On the contrary, HBOT alleviated the decrease of anti-apoptosis gene Bcl-2 and promoted the expression of neurotrophic factors (NTFs), such as NGF, BDNF, GDNF and NT-3 in vivo. As a consequent, the neuropathogenesis was remarkably relied with HBOT. Astrocytes from TBI brain or those cultured with 21% O2 density expressed higher NTFs than that of corresponding controls, from sham brain and cultured with 7% O2, respectively. The NTFs expression was the highest in astrocytes form TBI brain and cultured with 21% O2, suggesting a synergistic effect existed between TBI and the following HBO treatment in astrocytes. <br><br>CONCLUSION: Our findings provided evidence for the clinical usage of HBO treating brain damages.<p />  <p>Language: en</p>
LA  - en
SN  - 0102-8650
UR  - http://dx.doi.org/10.1590/s0102-865020180040000006
ID  - ref1
ER  -