TY  - JOUR
PY  - 2017//
TI  - Mass spectrometric imaging of ceramides tracks therapeutic response in traumatic brain injury
JO  - ACS chemical neuroscience
A1  - Barbacci, Damon Christopher
A1  - Roux, Aurelie
A1  - Muller, Ludovic
A1  - Jackson, Shelley N.
A1  - Post, Jeremy
A1  - Baldwin, Kathrine
A1  - Hoffer, Barry
A1  - Balaban, Carey
A1  - Gouty, Shawn
A1  - Cox, Brian M.
A1  - Woods, Amina S.
SP  - 2266
EP  - 2274
VL  - 8
IS  - 10
N2  - Traumatic brain injury (TBI) is a serious public health problem and the leading cause of death in children and young adults. It also contributes to a substantial number of cases of permanent disability. As lipids make up over 50% of the brain mass and play a key role in both membrane structure and cell signaling, their profile is of particular interest. In this study, we show that advanced mass spectrometry imaging (MSI) has sufficient technical accuracy and reproducibility to demonstrate the anatomical distribution of 50 micron diameter microdomains that show changes in brain ceramide levels in a rat model of controlled cortical impact (CCI) 3 days post injury with and without treatment. Adult male Sprague-Dawley rats received one strike and were euthanized 3 days post trauma. Brain MS images showed increase in ceramides in CCI animals compared to sham as well as significant reduction in ceramides in CCI treated animals demonstrating therapeutic effect of a peptide agonist. The data also suggests the presence of diffuse changes outside of the injured area. These results shed light on the extent of biochemical and structural changes in the brain after traumatic brain injury and could help to evaluate the efficacy of treatments.<p />  <p>Language: en</p>
LA  - en
SN  - 1948-7193
UR  - http://dx.doi.org/10.1021/acschemneuro.7b00189
ID  - ref1
ER  -