TY  - JOUR
PY  - 2018//
TI  - Identification and quantification of predominant metabolites of synthetic cannabinoid MAB-CHMINACA in an authentic human urine specimen
JO  - Drug testing and analysis
A1  - Hasegawa, Koutaro
A1  - Minakata, Kayoko
A1  - Gonmori, Kunio
A1  - Nozawa, Hideki
A1  - Yamagishi, Itaru
A1  - Watanabe, Kanako
A1  - Suzuki, Osamu
SP  - 365
EP  - 371
VL  - 10
IS  - 2
N2  - An autopsy case in which the cause of death was judged as drug poisoning by two synthetic cannabinoids, including MAB-CHMINACA, was experienced. Although unchanged MAB-CHMINACA could be detected from solid tissues, blood and stomach contents in the case, the compound could not be detected from the urine specimen. In this article, we obtained six kinds of reference standards of MAB-CHMINACA metabolites from a commercial source. The MAB-CHMINACA metabolites from the urine specimen of the abuser were extracted by a QuEChERS method including dispersive solid-phase extraction, and analyzed by liquid chromatography-tandem mass spectrometry with or without hydrolysis with β-glucuronidase. Among the six MAB-CHMINACA metabolites tested, 2 predominant metabolites could be identified and quantified in the urine specimen of the deceased. After hydrolysis with β-glucuronidase, increase of the two metabolites was not observed. The metabolites detected were a 4-monohydroxycyclohexylmethyl metabolite M1 (N-(1-amino-3,3-dimethyl-1-oxobutan-2-yl)-1-((4-hydroxycyclohexyl)methyl)-1H-indazole-3-carbo-xamide) of MAB-CHMINACA, and a dihydroxyl (4-hydroxycyclohexylmethyl and tert-butyl hydroxyl) metabolite M11 (N-(1-amino-4-hydroxy-3,3-dimethyl-1-oxobutan-2-yl)-1-((4-hydroxycyclohexyl)methyl)-1H-indaz-ole-3-carboxamide). Their concentrations were 2.17±0.15 and 10.2±0.3 ng/mL (n = 3, each) for M1 and M11, respectively. Although there is one preceding in vitro study showing the estimation of metabolism of MAB-CHMINACA using human hepatocytes, this is the first report dealing with in vivo identification and quantification of MAB-CHMINACA metabolites in an authentic human urine specimen.<br><br>This article is protected by copyright. All rights reserved.<p />  <p>Language: en</p>
LA  - en
SN  - 1942-7603
UR  - http://dx.doi.org/10.1002/dta.2220
ID  - ref1
ER  -