TY  - JOUR
PY  - 2016//
TI  - 18FFDG-PET combined with MRI elucidates the pathophysiology of traumatic brain injury in rats
JO  - Journal of neurotrauma
A1  - Brabazon, Fiona
A1  - Wilson, Colin M.
A1  - Shukla, Dinesh
A1  - Mathur, Sanjeev
A1  - Jaiswal, Shalini
A1  - Bermudez, Sara
A1  - Byrnes, Kimberly
A1  - Selwyn, Reed G.
SP  - 1074
EP  - 1085
VL  - 34
IS  - 5
N2  - Non-invasive measurements of brain metabolism using [18F]-fluorodeoxyglucose (FDG) with positron emission tomography (PET) may provide important information about injury severity following traumatic brain injury (TBI). There is growing interest in the potential of combining functional PET imaging with anatomical and functional magnetic resonance imaging (MRI). This study aimed to investigate the effectiveness of combining clinically available FDG-PET with T2 and diffusion MR imaging, with a particular focus on inflammation and the influence of glial alterations after injury. Adult male Sprague Dawley rats underwent a moderate controlled cortical impact (CCI) injury followed by FDG-PET, MRI and histological evaluation. FDG uptake showed significant alterations in the corpus callosum, hippocampus and amygdala after TBI, demonstrating that a relatively 'focal' CCI injury can result in global alterations. Analysis of MRI T2 intensity and apparent diffusion coefficient (ADC) also showed significant alterations in these regions to include cytotoxic and vasogenic edema. Histology showed increased glial activation in the corpus callosum and hippocampus that was associated with increased FDG uptake at sub-acute time points. Glial activation was not detected in the amygdala but neuronal damage was evident, as the amygdala was the only region to show a reduction in both FDG uptake and ADC at sub-acute time points. Overall, FDG-PET detected glial activation but was confounded by the presence of cell damage, while MRI consistently detected cell damage but was confounded by glial activation. These results demonstrate that FDG-PET and MRI can be used together to improve our understanding of the complex alterations in the brain after TBI.<p />  <p>Language: en</p>
LA  - en
SN  - 0897-7151
UR  - http://dx.doi.org/10.1089/neu.2016.4540
ID  - ref1
ER  -