TY  - JOUR
PY  - 2015//
TI  - Identification and quantification of metabolites of AB-CHMINACA in a urine specimen of an abuser
JO  - Legal medicine (Elsevier)
A1  - Wurita, Amin
A1  - Hasegawa, Koutaro
A1  - Minakata, Kayoko
A1  - Gonmori, Kunio
A1  - Nozawa, Hideki
A1  - Yamagishi, Itaru
A1  - Suzuki, Osamu
A1  - Watanabe, Kanako
SP  - 113
EP  - 118
VL  - 19
IS  - 
N2  - We experienced an autopsy case in which the cause of death was judged as poisoning by multiple new psychoactive substances, including AB-CHMINACA, 5-fluoro-AMB and diphenidine [Forensic Toxicol. 33 (2015): 45-53]. Although unchanged AB-CHMINACA could be detected from 8 solid tissues, it could neither be detected from blood nor urine specimens. In this article, we obtained eight kinds of reference standards of AB-CHMINACA metabolites from a commercial source. The AB-CHMINACA metabolites from the urine specimen of the abuser were extracted by a modified QuEChERS method and analyzed by liquid chromatography-tandem mass spectrometry before and after hydrolysis with β-glucuronidase. Among the eight AB-CHMINACA metabolites tested, only 2 metabolites could be identified in the urine specimen of the deceased. After hydrolysis with β-glucuronidase, the concentrations of the two metabolites were not increased, suggesting that the metabolites were not in the conjugated forms. The metabolites detected were 4-hydroxycyclohexylmethyl AB-CHMINACA (M1), followed by N-[[1-(cyclohexylmethyl)-1H-indazol-3-yl]carbonyl]-l-valine (M3). Their concentrations were 52.8±3.44 and 41.3±5.04ng/ml (n=10) for M1 and M3, respectively. Although there is one preceding report showing the estimations of metabolism of AB-CHMINACA without reference standards, this is the first report dealing with exact identification using reference standards, and quantification of M1 and M3 in an authentic urine specimen.<p />  <p>Language: en</p>
LA  - en
SN  - 1344-6223
UR  - http://dx.doi.org/10.1016/j.legalmed.2015.07.011
ID  - ref1
ER  -