TY  - JOUR
PY  - 2015//
TI  - Cardiac and renal nitrosative-oxidative stress after acute poisoning by a nerve agent Tabun
JO  - Journal of environmental science and health. Part A toxic/hazardous substances and environmental engineering
A1  - Dimov, Dimo
A1  - Hadjiolova, Radka
A1  - Kanev, Kamen
A1  - Tomova, Radka
A1  - Michova, Anna
A1  - Todorov, Todor
A1  - Murdjev, Rumen
A1  - Boneva, Temenujka
A1  - Dimova, Ivanka
SP  - 824
EP  - 829
VL  - 50
IS  - 8
N2  - We hypothesized that Tabun poisoning, as well as other organophosphorous treatment, cause specific organs' oxidative changes that have not previously been substantiated investigated. In this regard, a marker for nitrosative-oxidative stress in the main haemodynamic organs (heart and kidney) could reveal the existence of such changes. In this study, for the first time we studied the nitrosative/oxidative stress in heart and kidney after acute Tabun (Ethyl N,N- Dimethylphosphoramidocyanidate) poisoning measuring by immunohistochemistry the expression of 3-nitrotyrosine-a marker for nitrosative-oxidative stress. We investigated nitrotyrozine expression in three different groups of animals (with at least 3 animals in each group): the first group was treated with 0.5 LD50 Tabun and organs were collected after 24 h; the second group received vehicle for the same period; in the third group a highly specific re-activator was applied immediately after Tabun application. Heart and kidney were collected after 24 h. The levels of nitrotyrozine production significantly increased (more than 3 times) in cardiomyocytes after Tabun. The application of re-activator slightly reduced these levels not reaching the basal heart levels. Nitrotyrozine expression in kidney increased more than 2 times after Tabun and application of re-activator did not change it significantly. In conclusion, our study evidently demonstrated that Tabun trigger oxidative-nitrosative stress in heart and kidney and these cellular effects should be protected by an additional anti-oxidant therapy, since acetylcholinesterase re-activator is not efficient in this manner.<p />  <p>Language: en</p>
LA  - en
SN  - 1093-4529
UR  - http://dx.doi.org/10.1080/10934529.2015.1019801
ID  - ref1
ER  -