TY  - JOUR
PY  - 2015//
TI  - Characterization of traumatic brain injury in human brains reveals distinct cellular and molecular changes in contusion and pericontusion
JO  - Journal of neurochemistry
A1  - Harish, G.
A1  - Mahadevan, Anita
A1  - Pruthi, Nupur
A1  - Sreenivasamurthy, Sreelakshmi K.
A1  - Puttamallesh, Vinuth N.
A1  - Keshava Prasad, T. S.
A1  - Shankar, S. K.
A1  - Srinivas Bharath, Muchukunte Mukunda
SP  - 156
EP  - 172
VL  - 134
IS  - 1
N2  - Traumatic brain injury (TBI) contributes to fatalities and neurological disabilities worldwide. While primary injury causes immediate damage, secondary events contribute to long-term neurological defects. Contusions (Ct) are primary injuries correlated with poor clinical prognosis, and can expand leading to delayed neurological deterioration. Pericontusion (PC) (penumbra), the region surrounding Ct, can also expand with oedema, increased intracranial pressure, ischemia and poor clinical outcome. Analysis of Ct and PC can therefore assist in understanding the pathobiology of TBI and its management. The current study on human TBI brains noted extensive neuronal, astroglial and inflammatory changes, alterations in mitochondrial, synaptic and oxidative markers and associated proteomic profile, with distinct differences in Ct and PC. While Ct displayed petechial haemorrhages, thrombosis, inflammation, neuronal pyknosis and astrogliosis, PC revealed oedema, vacuolation of neuropil, axonal loss and dystrophic changes. Proteomic analysis demonstrated altered immune response, synaptic and mitochondrial dysfunction, among others, in Ct, while PC displayed altered regulation of neurogenesis and cytoskeletal architecture, among others. TBI brains displayed oxidative damage, glutathione depletion, mitochondrial dysfunction and loss of synaptic proteins, with these changes being more profound in Ct. We suggest that analysis of markers specific to Ct and PC may be valuable in the evaluation of TBI pathobiology and therapeutics. This article is protected by copyright. All rights reserved.<p />  <p>Language: en</p>
LA  - en
SN  - 0022-3042
UR  - http://dx.doi.org/10.1111/jnc.13082
ID  - ref1
ER  -