TY  - JOUR
PY  - 2014//
TI  - A threshold shear force for calcium influx in an astrocyte model of traumatic brain injury
JO  - Journal of neurotrauma
A1  - Maneshi, Mohammad Mehdi
A1  - Sachs, Frederick
A1  - Hua, Susan Z.
SP  - 1020
EP  - 1029
VL  - 32
IS  - 13
N2  - Traumatic brain injury (TBI) refers to brain damage resulting from external mechanical force such as a blast or crash. Our current understanding of TBI is derived mainly from in vivo studies that show measurable biological effects on neurons sampled after TBI. Little is known about the early responses of brain cells during stimuli and which features of the stimulus are most critical to cell injury. We generated defined shear stress in a microfluidic chamber using a fast pressure servo and examined the intracellular Ca2+ levels in cultured adult astrocytes. Shear stress increased intracellular Ca2+ depending on the magnitude, duration and the rise time of the stimulus. Square pulses with a fast rise time (~ 2 ms) caused transient increases in intracellular Ca2+, but when the rise time was extended to 20 ms, the response was much less. The threshold for a response is a matrix of multiple parameters. Cells can integrate the effect of shear force from repeated challenges: a pulse train of ten narrow pulses (11.5 dyn/cm2 and 10 ms wide) resulted in a four-fold increase in Ca2+ relative to a single pulse of the same amplitude 100 ms wide. The Ca2+ increase was eliminated in Ca2+-free media, but was observed after depleting the intracellular Ca2+ stores with thapsigargin suggesting the need for a Ca2+ influx. The Ca2+ influx was inhibited by extracellular Gd3+, a non-specific inhibitor of mechanosensitive ion channels (MSCs), but it was not affected by the more specific inhibitor GsMTx4. The voltage gated channel blockers, nifedipine, diltiazem, and verapamil were also ineffective. The data shows that the mechanically induced Ca2+ influx commonly associated with neuron models for TBI is also present in astrocytes, and there is a viscoelastic/plastic coupling of shear stress to the Ca2+ influx. The site of Ca2+ influx has yet to be determined.<p /> <p>Language: en</p>
LA  - en
SN  - 0897-7151
UR  - http://dx.doi.org/10.1089/neu.2014.3677
ID  - ref1
ER  -