TY  - JOUR
PY  - 2013//
TI  - Verification of chemical warfare agent exposure in human samples
JO  - Toxichem Krimtech
A1  - Elsinghorst, Paul W.
A1  - Thiermann, Horst
A1  - Koller, Marianne
SP  - 288
EP  - 292
VL  - 80
IS  - Spec Iss
N2  - AIM: This brief presentation provides an overview of methods that have been developed for the verification of human exposure to chemical warfare agents. <br><br>METHODS: GC-MS detection of nerve agents (V- and G-type) has been carried out with respect to unreacted agents as well as enzyme-bound species and metabolites. <br><br>METHODS involving direct SPE from plasma, fluoride-induced release of protein-bound nerve agents in plasma and analysis of their metabolites in plasma and urine have been developed. Exposure to blistering agents, i.e., sulfur mustard, has been verified by GC-MS detection of the unreacted agent in plasma and by LC- and GC-MS analysis of its metabolites in urine. <br><br>RESULTS: After incorporation nerve agents quickly bind to proteins, e.g., acetylcholinesterase, butyrylcholinesterase or serum albumin, and only small parts remain freely circulating for a few hours (G-type) or up to 2 days (V-type). Concurrently they are converted to O-alkyl methylphosphonic acids by phosphotriesterases and/or simply by aqueous hydrolysis. As a re- sult, different biomarkers can be detected depending on the time passed between exposure and sampling. Unreacted V-type agents can be detected in plasma for 2 days, the O-alkyl methyl- phosphonic acids in plasma for about 2-4 days and in urine for up to 1 week. Fluoride-indu- ced release of protein-bound nerve agents can be carried out until 3 weeks post exposure. Unmetabolized sulfur mustard may be detected between 8 hours to 8 weeks in plasma, while no time frame has been reported for its metabolites in urine. <br><br>CONCLUSION: A set of validated techniques detecting exposure to chemical warfare agents has been established. Further methods are needed to provide a clear picture of their biomarkers.<p />  <p>Language: en</p>
LA  - en
SN  - 2190-3441
UR  - http://dx.doi.org/
ID  - ref1
ER  -