TY  - JOUR
PY  - 1996//
TI  - Application of a solid-phase microextraction technique for the detection of urinary methamphetamine and amphetamine by gas chromatography
JO  - Journal (Canadian Society of Forensic Science)
A1  - Ameno, K.
A1  - Fuke, C.
A1  - Ameno, S.
A1  - Kinoshita, H.
A1  - Ijiri, I.
SP  - 43
EP  - 48
VL  - 29
IS  - 2
N2  - We describe here a simple and rapid detection of methamphetamine (MA) and amphetamine (AP) simultaneously in urine by gas chromatography (GC) using a solid-phase micro-extraction (SPME) technique. We used an SPME fiber (Polydimethylsiloxane thickness; 100μM) with a fiber holder for manual use. After addition of urine (1.0 ml), NaCI(0.5g) and 1-phenylbutylamine (5.0 μg, 1-PB, internal standard) into a vial (1.5 ml), we adjusted the sample to pH12 with NaOH (10N) and then rapidly sealed it with a teflon-coated septum. After immersion of the SPME fiber in the sample for 20 min, and then washing promptly in NaOH-H3BO4 buffer (pH12), the SPME needle was inserted into the injection port of the GC and the fiber extruded. Each peak of MA, AP and 1-PB was clearly separated. Linearity of AP and MA were in the ranges of 0.25-10 and 0.1-1.0 μg/ml, respectively. This SPME technique, applicable without using organic solvents, is simple and rapid. It is, therefore, useful for the practical detection of MA and AP in urine.<p />
LA  - en
SN  - 0008-5030
UR  - http://dx.doi.org/10.1080/00085030.1996.10757046
ID  - ref1
ER  -