TY - JOUR
PY - 2013//
TI - Proteomic comparison of Hypnale hypnale (Hump-Nosed Pit-Viper) and Calloselasma rhodostoma (Malayan Pit-Viper) venoms
JO - Journal of Proteomics
A1 - Ali, Syed A.
A1 - Baumann, Kate
A1 - Jackson, Timothy N. W.
A1 - Wood, Kelly
A1 - Mason, Steve
A1 - Undheim, Eivind A. B.
A1 - Nouwens, Amanda
A1 - Koludarov, Ivan
A1 - Hendrikx, Iwan
A1 - Jones, Alun
A1 - Fry, Bryan Grieg
SP - 338
EP - 343
VL - 91
IS -
N2 - Treatment of Hypnale hypnale bites with commercial antivenoms, even those raised against its sister taxon Calloselasma rhodostoma, have never been clinically successful. As these two genera have been separated for 20 million years, we tested to see whether significant variations in venom had accumulated during this long period of evolutionary divergence, and thus could be responsible for the failure of antivenom. Proteomic analyses of C. rhodostoma and H. hypnale venom were performed using 1D and 2D PAGE as well as 2D-DIGE. C. rhodostoma venom was diverse containing large amounts of Disintegrin, Kallikrein, L-amino acid oxidase, Lectin, phospholipase A2 (acidic, basic and neutral) and Snake Venom Metalloprotease. In contrast, while H. hypnale also contained a wide range of toxin types, the venom was overwhelmingly dominated by two molecular weight forms of basic PLA2. 2D-DIGE (2-D Fluorescence Difference Gel Electrophoresis analysis) showed that even when a particular toxin class was shared between the two venoms, there were significant molecular weight or isoelectric point differences. This proteomic difference explains the past treatment failures with C. rhodostoma antivenom and highlights the need for a H. hypnale specific antivenom. BIOLOGICAL SIGNIFICANCE: These results have direct implications for the treatment of envenomed patients in Sri Lanka. The unusual venom profile of Hypnale hypnale underscores the biodiscovery potential of novel snake venoms. Language: en
LA - en
SN - 1874-3919
UR - http://dx.doi.org/10.1016/j.jprot.2013.07.020
ID - ref1
ER -