TY  - JOUR
PY  - 2013//
TI  - Neonate-to-adult transition of snake venomics in the short-tailed pit viper, Gloydius brevicaudus
JO  - Journal of Proteomics
A1  - Gao, Jian-Fang
A1  - Qu, Yan-Fu
A1  - Zhang, Xiu-Qin
A1  - He, Ying
A1  - Ji, Xiang
SP  - 148
EP  - 157
VL  - 84
IS  - 
N2  - Snake venoms undergo ontogenetic shifts in biochemical and pharmacological activities. This may be related to variation in venom components associated with the ontogenetic shift in diet. We used the short-tailed pit viper Gloydius brevicaudus that displays ontogenetic shifts in diet to examine whether the species displays a neonate-to-adult transition of snake venomics. Venoms from neonates and adults were pooled separately and then analyzed by 2-DE, MALDI-TOF-MS/MS and iTRAQ technologies. The 2-DE profiles showed that the main components in both types of venoms were acidic proteins, and that neonates and adults differed in snake venomics. The proteins with molecular masses/pI of ~12-39kDa/4.0-4.6, ~36-57kDa/5.6-7.0 and ~65-92kDa/4.5-5.8 were more abundant in the neonate venom, while the proteins with molecular masses/pI of ~12-19kDa/4.6-6.4, ~23-30kDa/5.4-6.3 and ~35-62kDa/4.6-5.4 were more abundant in the adult venom. The iTRAQ analysis showed quantitative changes in various toxin families, including mainly metalloproteinases, serine proteinases, phospholipase A2s and C-type lectins. The N-deglycosylation analysis demonstrated that glyocosylation was an important post-translational modification of snake venom. Our results show a neonate-to-adult transition of snake venomics in G. brevicaudus. Such a transition might be driven by the divergence in dietary habits between neonates and adults. Biological Significance This study is first to demonstrate a neonate-to-adult transition of snake venomics in G. brevicaudus, and the results will be helpful in predicting and treating clinical pathologic symptoms caused by the snake at different developmental stages.<p />  <p>Language: en</p>
LA  - en
SN  - 1874-3919
UR  - http://dx.doi.org/10.1016/j.jprot.2013.04.003
ID  - ref1
ER  -