TY  - JOUR
PY  - 2013//
TI  - Why myotoxin-containing snake venoms possess powerful nucleotidases?
JO  - Biochemical and biophysical research communications
A1  - Caccin, Paola
A1  - Pellegatti, Patrizia
A1  - Fernandez, Julian
A1  - Vono, Maria
A1  - Cintra-Francischinelli, Mariana
A1  - Lomonte, Bruno
A1  - Gutiérrez, José María
A1  - Di Virgilio, Francesco
A1  - Montecucco, Cesare
SP  - 1289
EP  - 1293
VL  - 430
IS  - 4
N2  - The venom of the snake Bothrops asper causes muscle necrosis, pain and inflammation. This venom contains myotoxins which cause an increase in intracellular Ca(2+) concentration and release of K(+) and ATP from myotubes. ATP is a key danger molecule that triggers a variety of reactions, including activation of the innate immune response. Here, using ATP-luciferase bioluminescence imaging technique, we show for the first time in vivo, that the purified myotoxins induce rapid release of ATP, whilst the complete venom of B. asper does at a very small extent. This apparent contradiction is explained by the finding that the venom contains powerful nucleotidases that in vivo convert ATP into ADP, AMP and Adenosine. These findings indicate that high concentrations of adenosine are generated by the double action of the venom and provide the experimental basis to the suggestion that in situ generated adenosine plays an important role in envenomation via its hypotensive, paralyzing and anti-coagulant activities.<p />  <p>Language: en</p>
LA  - en
SN  - 0006-291X
UR  - http://dx.doi.org/10.1016/j.bbrc.2012.11.129
ID  - ref1
ER  -