TY  - JOUR
PY  - 2011//
TI  - Altered expression pattern of testican-1 mRNA after brain injury
JO  - Biomedical research (Tokyo, Japan)
A1  - Iseki, Ken
A1  - Hagino, Seita
A1  - Zhang, Yuxiang
A1  - Mori, Tetsuji
A1  - Sato, Nobuko
A1  - Yokoya, Sachihiko
A1  - Hozumi, Yasukazu
A1  - Goto, Kaoru
A1  - Tase, Choichiro
SP  - 373
EP  - 378
VL  - 32
IS  - 6
N2  - Testican, a chondroitin/heparan sulfate proteoglycan, is primarily expressed in neurons of the adult and embryonic mouse brain, suggesting its role in normal and/or proliferation and differentiation processes of neurons. However, the role of testican in injured brain remains unclear. In the present study we investigated testican-1 mRNA expression pattern after cryo-injury of the brain. In situ hybridization histochemistry revealed that testican-1 mRNA is induced in the region surrounding the necrotic tissue. Time course study of testican-1 mRNA showed the highest level of signal intensity at 7 days after the injury. To determine which cell types express testican-1 mRNA, we performed in situ hybridization histochemistry combined with immunohistochemistry of several cell markers. Testican-1 mRNA signals were detected in the proximal reactive astrocytes, whereas the distribution pattern of testican-1 mRNA positive cells was different from those of mature oligodendrocytes and activated microglia. In addition, signals for testican-1 mRNA overlapped with those of FGF-2 mRNA, showing that these molecules are coexpressed in reactive astrocytes. These results suggest a possibility that testican-1 plays a permissive role for regenerating axons in reactive astrocytes after injury.<p />  <p>Language: en</p>
LA  - en
SN  - 0388-6107
UR  - http://dx.doi.org/
ID  - ref1
ER  -