TY  - JOUR
PY  - 1986//
TI  - A new method for the determination in blood and urine of a novel triazolobenzodiazepine (estazolam) by HPLC
JO  - Journal of analytical toxicology
A1  - Scotto di Tella, A.
A1  - Ricci, P.
A1  - Di Nunzio, C.
A1  - Cassandro, P.
SP  - 65
EP  - 67
VL  - 10
IS  - 2
N2  - In this study the authors report a new method to determine estazolam in blood and urine by high pressure liquid chromatography (HPLC). This compound is a triazolobenzodiazepine and shows an interesting psychopharmacological activity. The extraction from biological fluids was carried out using a mixture of ethylenechloride, methylenechloride, and ethylacetate (1:1:8). The HPLC analysis was carried out with a C18 column, using methanol:phosphate buffer (pH 7.5, 0.011M):acetonitrile (65:33:2) as mobile phase. The detector was set at lambda = 240 nm. The method shows good repeatability and a linear response in the range of 0.6 to 10 micrograms estazolam/mL both in serum and urine. A case of non-lethal acute intoxication in which the method was applied is also reported.<p />  <p>Language: en</p>
LA  - en
SN  - 0146-4760
UR  - http://dx.doi.org/
ID  - ref1
ER  -