TY  - JOUR
PY  - 2008//
TI  - A fatal case of bupropion (Zyban) overdose
JO  - Journal of analytical toxicology
A1  - Mercerolle, Magali
A1  - Denooz, Raphael
A1  - Lachâtre, Gérard
A1  - Charlier, C.
SP  - 192
EP  - 196
VL  - 32
IS  - 2
N2  - A sensitive and reproducible method for the identification and the quantitative determination of bupropion (BUP) and its major metabolites, hydroxybupropion (OH-BUP) and threohydrobupropion (T-BUP), was developed in blood and urine. The three compounds were extracted with a solid-phase extraction procedure followed by LC-ESI-MS-MS separation and quantification using decadeuterated lidocaine as internal standard. BUP and its metabolites were satisfactorily identified by multiple reactions monitoring detection. The limits of detection and quantification were determined at 5 and 10 microg/L, respectively, for each analyte. The intraday and interday coefficients of variability were lower than 11.9% for BUP and its metabolites. This method was applied to the forensic case of a 35-year-old male who died after a suspected ingestion of 30 slow-release tablets of Zyban. As samplings were performed at least 72 h after the drug intake, BUP had disappeared from blood, but OH-BUP and T-BUP were present at the concentrations of 5.8 and 30.4 mg/L, respectively. In urine, concentrations ranged from 42.9 mg/L for BUP to 617 mg/L for T-BUP. These results agree with the hypothesis of a successful suicide attempt.<p />  <p>Language: en</p>
LA  - en
SN  - 0146-4760
UR  - http://dx.doi.org/
ID  - ref1
ER  -