%0 Journal Article
%T Endogenous interleukin 10 prevents apoptosis in macrophages during salmonella infection
%J Biochemical and biophysical research communications
%D 1995
%A Arai, T.
%A Hiromatsu, K.
%A Nishimura, H.
%A Kimura, Y.
%A Kobayashi, N.
%A Ishida, H.
%A Nimura, Y.
%A Yoshikai, Y.
%V 213
%N 2
%P 600-607
%X To elucidate the biological roles of endogenous interleukin 10 (IL-10) in macrophage responses during bacterial infection, we examined in vitro effects of neutralizing IL-10 by anti-IL-10 monoclonal antibodies (mAb) on apoptosis of the peritoneal macrophages following Salmonella choleraesuis infection. Marked increments of TNF-α production were observed in the culture supernatant later than 6 h after in vitro culture with anti-IL-10 mAb. These macrophages succumbed to apoptosis at this stage accompanied by marked increment of IL-1 release, despite the expression of higher amount of endogenous heat shock protein 70, an inhibitor of TNF-α-mediated apoptosis. These results suggest that endogenous IL-10 plays an essential role in protection of Salmonella-infected macrophages from autocrine suicide caused by excessive production of TNF-α after killing of Salmonella. © 1995 by Academic Press, Inc.<p /><p>Language: en</p>
%G en
%I Academic Press
%@ 0006-291X
%U http://dx.doi.org/10.1006/bbrc.1995.2174