%0 Journal Article
%T Effect of ultrasound microbubble carrying herpes simplex virus thymidine kinase on hepatocellular carcinoma in mice
%J Zhonghua gan zang bing za zhi
%D 2010
%A Zhou, Shi-ji
%A Liu, Chan-an
%A Gong, Jian-ping
%A Liu, Zuo-jin
%A Tang, Yong
%A Li, Sheng-wei
%A Xu, Yue
%A Ai, Zhi-guo
%V 
%N 12
%P 276-279
%X &lt;p&gt;&lt;b&gt;OBJECTIVE&lt;/b&gt;To observe the effect of ultrasound microbubble carrying herpes simplex virus thymidine kinase hepatocellular carcinoma in mice.&lt;/p&gt;&lt;p&gt;&lt;b&gt;METHODS&lt;/b&gt;Kunming mice were inoculated subcutaneously with H22 tumor cells. 40 male mice bearing subcutaneous hepatoma were randomized into 4 groups: PBS (group A), HSV1-TK (group B), HSV1-TK (group C), and microbubble carrying HSV1-TK (group D) were injected into the tail vein every 3 days. Mice in group C and D were exposed to ultrasound. The expression of TK protein was detected by western blot. Ganciclovir (GCV) was intraperitoneally injected at a dose of 100 mg x kg (-1) x d(-1) in group B, group C and group D. The tumor size was measured every 2 days.&lt;/p&gt;&lt;p&gt;&lt;b&gt;RESULTS&lt;/b&gt;TK gene could be injected precisely into hepatocellular carcinoma with ultrasound monitor, and the expression of TK protein was found in all 4 groups. Expression in group D was higher than others (P &lt; 0.05). The rate of tumor growth inhibition were 0 in group A, 3.90%+/-1.80% in group B, 22.70%+/-2.86% in group C, 41.25%+/-3.20% in group D (group B vs group C, P &lt; 0.05; group D vs group C, P &lt; 0.05; group D vs group B, P &lt; 0.05).&lt;/p&gt;&lt;p&gt;&lt;b&gt;CONCLUSION&lt;/b&gt;Ultrasound microbubble not only improve target gene therapy, but also enhance transfection efficiency.&lt;/p&gt;<p /><p>Language: zh</p>
%G zh
%I Chongqing yi ke da xue di er lin chuang xue yuan bing du xing gan yan yan jiu suo
%@ 1007-3418
%U http://dx.doi.org/10.3760/cma.j.issn.1007-3418.2010.04.010