%0 Journal Article
%T Experimental study of burn damage progression in a human composite tissue model
%J Biology (Basel)
%D 2021
%A Hao, Dandan
%A Nourbakhsh, Mahtab
%A Qu, Miao
%V 10
%N 1
%P e40-e40
%X Comparative studies of human tissue damage caused by burns are challenging because precise information regarding the temperature, time, and duration of the exposure is  often missing. Animal models cannot be fully translated to the human system due to  interspecies differences in cutaneous tissues. We used a human composite tissue  model to compare tissue damage caused by thermal burns with different dynamics. Equal subcutaneous/cutaneous composite tissue samples from six donors were first  exposed to either preheated steel (100 °C) or a precision flame burner (300 °C) and  were then maintained in vitro for seven days. Histological and immunohistochemical  analyses revealed that flame burns instantly caused deep and stable damage to the  subcutaneous tissue, which stayed constant for seven days. By contrast, contact  burns inflicted tissue damage that was initially superficial but then expanded  deeper into the adipose tissue. This spatiotemporal expansion of tissue damage was  essentially accompanied by macrophage and fibroblast activation, which points  towards inflammation resolution and wound healing. Our study suggests that thermal  differences in burns directly influence the course of tissue damage, the cellular  response and, consequently, the likely dynamics of repair processes days after burn  injuries.<p /> <p>Language: en</p>
%G en
%I MDPI: Multidisciplinary Digital Publications Institute
%@ 2079-7737
%U http://dx.doi.org/10.3390/biology10010040