%0 Journal Article
%T Persian Gulf jellyfish (Cassiopea andromeda) venom fractions induce selective injury and cytochrome C release in mitochondria obtained from breast adenocarcinoma patients
%J Asian Pacific journal of cancer prevention
%D 2017
%A Mirshamsi, Mohammad Reza
%A Omranipour, Ramesh
%A Vazirizadeh, Amir
%A Fakhri, Amir
%A Zangeneh, Fatemeh
%A Mohebbi, Gholam Hussain
%A Seyedian, Ramin
%A Pourahmad, Jalal
%V 18
%N 1
%P 277-286
%X OBJECTIVE: This study was conducted to investigate whether fractions of jellyfish Cassiope andromeda venom, could selectively induce toxicity on mitochondria isolated from cancer tissue of patients with breast adenocarcinomas. <br><br>METHODS: Firstly, we extracted two fractions, (f1 and f2) from crude jellyfish venom by gel filtration on Sephadex G-200.Then different dilutions of these extracted fractions were applied to mitochondria isolated from human breast tumoral- and extra-tumoral tissues. Parameters of mitochondrial toxicity including generation of reactive oxygen species (ROS), mitochondrial membrane potential (MMP) collapse, swelling, cytochrome c release, activation of caspase3 and apoptosis were then assayed. <br><br>RESULT: Our results demonstrate that fraction 2 of Cassiopea andromeda crude venom significantly (P<0.05) decreased mitochondrial succinate dehydrogenase activity, increased mitochondrial ROS production, induced mitochondrial swelling, MMP collapse and cytochrome c release, activated caspase3 and induced apoptosis only in tumoral mitochondria, and not in mitochondria obtained from extra-tumoral tissue (P<0.05). <br><br>CONCLUSION: In conclusion this study suggested that fraction 2 of Cassiopea andromeda crude venom selectively induces ROS mediated cytotoxicity by directly targeting mitochondria isolated from human breast tumor tissue.<br><br>Creative Commons Attribution License<p /> <p>Language: en</p>
%G en
%I Asian Pacific Organization for Cancer Prevention
%@ 2476-762X
%U http://dx.doi.org/10.22034/APJCP.2017.18.1.277