%0 Journal Article
%T A new method for the determination in blood and urine of a novel triazolobenzodiazepine (estazolam) by HPLC
%J Journal of analytical toxicology
%D 1986
%A Scotto di Tella, A.
%A Ricci, P.
%A Di Nunzio, C.
%A Cassandro, P.
%V 10
%N 2
%P 65-67
%X In this study the authors report a new method to determine estazolam in blood and urine by high pressure liquid chromatography (HPLC). This compound is a triazolobenzodiazepine and shows an interesting psychopharmacological activity. The extraction from biological fluids was carried out using a mixture of ethylenechloride, methylenechloride, and ethylacetate (1:1:8). The HPLC analysis was carried out with a C18 column, using methanol:phosphate buffer (pH 7.5, 0.011M):acetonitrile (65:33:2) as mobile phase. The detector was set at lambda = 240 nm. The method shows good repeatability and a linear response in the range of 0.6 to 10 micrograms estazolam/mL both in serum and urine. A case of non-lethal acute intoxication in which the method was applied is also reported.<p /> <p>Language: en</p>
%G en
%I Preston Publications
%@ 0146-4760
%U http://dx.doi.org/