@article{ref1,
title="Specific site distribution of TN 5 in pseudomonas putida PpGI-MI using suicide plasmid pSUP 2021",
journal="Egypt. J. Microbiol.",
year="1991",
author="T.m.a., El Kawokgy",
volume="26",
number="3",
pages="443-451",
abstract="Transposon Tn 5, which confers kanamycin-neomycin resistance, was introduced into the genome of Pseudomonas putida PpG1-M1 by conjugation with Escherichia coli SM10 containing the suicide plasmid vector pSUP2021. Approximately, 1.82% of over 2200 neomycin resistant [Nmr] colonies were mutated to auxotrophy. Most auxotrophs reverted at low frequencies [<10-8] and most prototrophic revertants were neomycin sensitive. The Tn 5-induced auxotrophic mutations were mainly distributed among genes of aspartic acid group indicating specific site-distribution of Tn 5 among the genome of Pseudomonas putida PpG1-M1<p /><p>Language: en</p>",
language="en",
issn="0022-2704",
doi="",
url="http://dx.doi.org/"
}