@article{ref1,
title="Radiation-inducible promoters-mediated cdglytk gene in the treatment of buccal carcinoma in golden hamster",
journal="Chinese journal of stomatology",
year="2006",
author="Yu, Dong-sheng and Huang, Hong-zhang and Hu, Xiao-wen and Liu, Xi-qiang and Tang, Hai-kuo and Wang, An-xun",
volume="",
number="12",
pages="549-552",
abstract="&lt;p&gt;&lt;b&gt;OBJECTIVE&lt;/b&gt;To observe the therapeutic effect of CDglyTK gene mediated by radiation-inducible promoters in the treatment of buccal carcinoma in Golden Hamster.&lt;/p&gt;&lt;p&gt;&lt;b&gt;METHODS&lt;/b&gt;Animal models of buccal carcinoma in golden hamster were established by painting 0.5% dimethyl-benzanthracene. The plasmids pcDNA (+) 3.1/E-CDglyTK were transfected into tumors by lipofectamine. 24 h later, the tumors were exposed to 3 Gy irradiation. Animals were monitored at regular intervals for volume of tumors. CDglyTK mRNA was assayed by RT-PCR. Apoptosis and proliferating cell nuclear antigen were detected respectively by in situ end-labeling and immunohistochemical methods.&lt;/p&gt;&lt;p&gt;&lt;b&gt;RESULTS&lt;/b&gt;Compared with control groups, the tumor was suppressed obviously by CDglyTK gene therapy combined with 3 Gy induction radiation. The expression of CDglyTK gene could be detected by RT-PCR in the transfected tumor, and up-regulation of CDglyTK expression was found in tumor exposed to radiation (P &lt; 0.05). There was significant difference in apoptosis index or proliferation index between tumor without irradiation and tumor with irradiation (P &lt; 0.05).&lt;/p&gt;&lt;p&gt;&lt;b&gt;CONCLUSIONS&lt;/b&gt;The radiation-inducible promoter can be served as a molecular switch to regulate the expression of CDglyTK gene in buccal carcinoma in golden hamster, and low dose induction radiation can significantly improve the therapeutic effects.&lt;/p&gt;<p /><p>Language: zh</p>",
language="zh",
issn="1002-0098",
doi="",
url="http://dx.doi.org/"
}