@article{ref1,
title="pAdKDR-tk and pAdCMV suicide gene system cytotoxicity and specificity in vitro",
journal="Cancer Research and Clinic",
year="2006",
author="Qiu, Qianhui and Li, Tianying and Chen, Shaohua and Zhang, Siyi",
volume="",
number="6",
pages="-",
abstract="OBJECTIVE In order to identify cytotoxicity and specificity of the suicide gene systems which vascular endothelial growth factor receptor 2(KDR) and CMV were used as promoters respectively. <br><br>METHODS Two cell lines, HUVEC and CNE-2, one expresses KDR highly,another doesn't, were used as target cells for identifying the cytotoxicity and specificity of recombinant adenoviral vectors with herpes simplex virus thymidine kinase (HSV-tk) gene expression driven by KDR promoter and CMV promoter respectively (pAdKDR-tk and pAdCMV-tk) in Vitro by adding GCV. <br><br>RESULTS HUVEC and CNE-2 showed similarly sensitivity to GCV when they were transfected by pAdCMV-tk. However, They shown different sensitivity to GCV when they were transfected by pAdKDR-tk. When HUVEC was transfected in high MOI (MOI=100) and concentration of GCV was 50 ?g/ml, The cell growth rate decreased to (23.12?4.90)%.On the contrary, CNE-2 cells growth rate remained as high as (70.46 ?3.27)%. <br><br>CONCLUSION Two kinds of cell line CNE-2 and HUVEC can be killed by pAdCMV-tk suicide gene system, but pAdKDR-tk suicide gene system only can kill HUVEC cell line. The result implied that pAdKDR-tk could kill the cells that express KDR specifitly.<p /><p>Language: zh</p>",
language="zh",
issn="1006-9801",
doi="",
url="http://dx.doi.org/"
}