@article{ref1,
title="Preliminary effect of VEGF promoter-driven recombinant adenovirus containing double suicide genes on apoptosis of human gastric carcinoma cells",
journal="Nan Fang Yi Ke Da Xue Xue Bao",
year="2007",
author="Kong, Heng and Huang, Zong-Hai and Li, Zhou and Yu, Jing-Long and Chen, Hai-Jin and Han, Xin-Jun",
volume="",
number="12",
pages="1152-1160",
abstract="&lt;p&gt;&lt;b&gt;OBJECTIVE&lt;/b&gt;To study the effect of the adenovirus containing CD/TK fusion gene controlled by the human vascular endothelial growth factor (VEGF) promoter on apoptosis of human gastric carcinoma cells SGC-7901.&lt;/p&gt;&lt;p&gt;&lt;b&gt;METHODS&lt;/b&gt;VEGF-expressing SGC-7901 cells were infected by the recombinant adenovirus Ad-VEGFP-CD/TK, and the infection efficiencies were observed with fluorescence microscopy. The toxic effect and intracellular calcium concentration induced by 5-fluorocytosine (5-FC) and ganciclovic (GCV) were determined by light microscopy, electron microscopy and flow cytometry.&lt;/p&gt;&lt;p&gt;&lt;b&gt;RESULTS&lt;/b&gt;The transfection efficiency of the recombinant adenovirus in SGC-7901 cells increased with the viral titer. At the multiplicity of infection (MOI) of 100, 5-FC and GCV could induce apoptosis of SGC-7901 cells within a given dose range in a dose- and time-dependent manner, and apoptotic changes of the cells were observed with electron microscopy. Apoptotic peak was also detected by flow cytometry. Cell cycle analysis revealed increased cell percentage in G(0)-G(1) phase and decreased percentage of cells in G(2)-M and S phases in response to treatment with the pro-drugs, which also induced marked elevation of intracellular calcium concentration in the infected cells.&lt;/p&gt;&lt;p&gt;&lt;b&gt;CONCLUSIONS&lt;/b&gt;CD/TK fusion gene system driven by VECF promoter selectively induces apoptosis of VEGF-expressing SGC-7901 cells, the action of which is probably mediated by intracellular calcium variation.&lt;/p&gt;<p /><p>Language: zh</p>",
language="zh",
issn="1673-4254",
doi="",
url="http://dx.doi.org/"
}