@article{ref1,
title="Mechanism of DADS in the bystander effect of HSV-tk/GCV suicide gene therapy system in lens epithelial cells",
journal="Zhong Nan Da Xue Xue Bao Yi Xue Ban",
year="2011",
author="Li, Zhongqing and Tan, Qian and Ding, Zhixiang and Liu, Dan",
volume="",
number="12",
pages="329-334",
abstract="OBJECTIVE@#To investigate the mechanism and effect of diallyl disulfide (DADS) on the bystander effect of herpes simplex virus kinase/ganciclovir (HSV-tk/GCV) suicide gene therapy system which was mediated by recombinant adeno-associated virus (rAAV) in lens epithelial cells.@*METHODS@#Immunohistochemistry method was used to determine the distribution of connexin 43 (Cx43) protein in cultured rabbit lens epithelial cells and rabbit lens epithelial cells transfected by HSV-tk suicide gene. Cx43 protein was measured and analyzed in cultured rabbit lens epithelial cells and rabbit lens epithelial cells transfected by HSV-tk suicide gene that was induced by various DADS. Cell survival was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method.@*RESULTS@#DADS increased the Cx43 protein expression in cultured rabbit lens epithelial cells and rabbit lens epithelial cells transfected by HSV-tk suicide gene, and especially in 50 μmol/L DADS. After combining with DADS, the bystander effect was significantly improved (P<0.05).@*CONCLUSION@#DADS can elevate the expression of Cx43 protein and enhance the bystander effect of HSV-tk/GCV suicide gene therapy system.<p /><p>Language: zh</p>",
language="zh",
issn="1672-7347",
doi="10.3969/j.issn.1672-7347.2011.04.009",
url="http://dx.doi.org/10.3969/j.issn.1672-7347.2011.04.009"
}