@article{ref1,
title="Effects of cytosine deaminase and HSV-tk genes in combination on gallbladder cancer cells mediated by retroviral vector",
journal="Chinese journal of general surgery",
year="2001",
author="Chang, Xinzhong and Wang, Zhanming and Zhang, Yi and Li, Zhiwei and Zhao, Zhilun and Ma, Daoxin and Sun, Jiazhi",
volume="",
number="12",
pages="-",
abstract="OBJECTIVE To investigate cell-killing and bystander effects on gallbladder cancer (GBC-SD) cells transferred with double suicide genes (CD and HSV-tk). <br><br>METHODS CD and HSV-tk genes were transfected into PA317 cells using lipofectamine. GBC-SD cells were infected with viral supernatant. GBC-SD/CD+tk cells were treated with 5-FC and/or GCV. GBC-SD/CD+tk and GBC-SD were inoculated subcutaneously into the nude mice respectively and treated with GCV and 5-FC for 21 days. <br><br>RESULTS The killing effect of 5-FC and GCV in combination on GBC-SD/CD+tk cells is more effective than using 5-FC or GCV alone. The local bystander effect is significant while the distant bystander effect was not obvious. <br><br>CONCLUSION The cell-killing efficacy of chemotherapy on GBC-SD transfected with double suicide gene was significantly increased, and the bystander effect was obvious.<p /><p>Language: zh</p>",
language="zh",
issn="1007-631X",
doi="",
url="http://dx.doi.org/"
}