@article{ref1,
title="Efficacy of HSV-tk/GCV system on human laryngeal squamous cell cancer in vitro",
journal="Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi",
year="2006",
author="Ding, Xiu-yong and Qin, Yong and Li, Fu-ying and Cong, Tie-chuan",
volume="",
number="12",
pages="374-376",
abstract="&lt;p&gt;&lt;b&gt;OBJECTIVE&lt;/b&gt;Efficacy of HSV-tk/GCV system antitumor effects was assessed on human laryngeal cancer cell line Hep-2 in vitro. To assess the HSV-tk/CGV system whether has an antitumour effect on human laryngeal squamous cell cancer Hep-2 in vitro. The mechanisms of cytotoxity were also assessed.&lt;/p&gt;&lt;p&gt;&lt;b&gt;METHODS&lt;/b&gt;Hep-2 cells were transfected with HSV-tk gene by lipofection. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the HSV-tk gene expression. MTT was utilized to test for the cytotoxicity of this system. The cell-circle arrest and apoptosis were analyzed by flowcytometry assay.&lt;/p&gt;&lt;p&gt;&lt;b&gt;RESULTS&lt;/b&gt;HSV-tk gene transfected cells demonstrated obvious cytoreductivity followed by ganciclovir (GCV) administration and this cytoreductivity showed partial GCV dose-independent. HSV-tk gene transfected cells demonstrated obvious s-phase arrest, no apoptosis and necrosis occurred.&lt;/p&gt;&lt;p&gt;&lt;b&gt;CONCLUSIONS&lt;/b&gt;The HSV-tk/GCV system can inhabit the growth of Hep-2 cells effectively. S-phase arrest perhaps is the main reason that leads to the cell inhibition in our study. HSV-tk/GCV system has potential antitumor effects for the future clinical practice.&lt;/p&gt;<p /><p>Language: zh</p>",
language="zh",
issn="1673-0860",
doi="",
url="http://dx.doi.org/"
}