@article{ref1,
title="Contribution of motA gene in pathogenesis-associated chemotaxis and colonization of Campylobacter jejuni",
journal="Chinese Journal of Microbiology and Immunology",
year="2010",
author="Ruan, Ping and Sun, Aihua and Zhao, Xin and Yan, Jie",
volume="",
number="12",
pages="195-200",
abstract="OBJECTIVE To determine the role of flagellar motor protein MotA in the pathogenesisassociated chemotaxis and colonization of Campylobacter jejuni. <br><br>METHODS The motA gene as well as Kan~r gene and plus-motA gene segments for motA gene knock-out were amplified by PCR and the target amplification fragments were sequenced after cloning. A suicide plasmid(pBlueskrit-Ⅱ-SK~(motA-kan)) and a motA gene knock-out mutant (motA~-) were constructed based on homologious recombination. By using semisolid plate migration test, hard agar plus (HAP)-based chemotactic test towards sodium deoxycholate (SDC) in vitro, and jejunal colonization test in BALB/c-ByJ mice were performed to determine the differences of flagellar motility, chemotaxis towards SDC and colonization in murine jejunum between motA~- mutant and wild-type strain. <br><br>RESULTS The nucleotide and amino acid sequences of the cloned motA gene were 100% identical to the reported corresponding sequences. The results of PCR, sequencing and continuous passage culture in antibiotics-contained medium demonstrated that both suicide plasmid and motA~- mutant were successfully generated. The diameters of clonies on semisolid plate and 0.2 mol/L SDC-induced chemotactic tings in HAP as well as the bacterial numbers adhering to the surface of murine jejunal mucosa and in jejunal content of motA~- mutant were significantly less than those of wild-type strain(P&lt;0.05). <br><br>CONCLUSION A motA gene knock-out mutant of C. jejuni was successfully constructed in this study, motA is an essential gene for flagellax motility, pathogenesis-associated chemotaxis and colonization of C. jejuni.<p /><p>Language: zh</p>",
language="zh",
issn="0254-5101",
doi="10.3760/cma.j.issn.0254-5101.2010.03.001",
url="http://dx.doi.org/10.3760/cma.j.issn.0254-5101.2010.03.001"
}