@article{ref1,
title="Construction of Salmonella Pullorum C79-13ΔcrpΔasd mutant balanced-lethal host-vector system and its biological features",
journal="Chinese Journal of Immunology",
year="2014",
author="Chen, Songbiao and Li, Jing and Shang, Ke and Yu, Chuan and Zhang, Chunjie and Cheng, Xiangchao and Li, Yinju and Zhao, Zhanqin",
volume="",
number="12",
pages="1083-1087,1092",
abstract="In order to develop a safer vaccine strain exploit Salmonella Pullorum vaccine strain as a live vaccine vector.<br><br>METHODS:AΔcrpΔasd mutant of S.pullorum C79-13 strain was constructed and it was developed E.coli donor strain mutant was generated through the two-step method introduced by χ7213 ( pREΔasd) was conjugated with the recipient of C 79-13Δcrp.The C79-13ΔcrpΔasd the transduction of recombinant suicide plasmid.<br><br>RESULTS:PCR and sequencing results showed that ΔsipBSL1344 was suc-cessfully constructed.The further study indicated that foreign DAP must be supplied for the ΔcrpΔasd mutant to grow,in addition,the asd gene was transmitted stably.Compared with C79-13 strain,the O antigens was identical to C79-13 strain,but the growth velocity was reduced significantly ,significantly reduced virulence.<br><br>CONCLUSION: The ΔcrpΔasd mutant can accept asd+plasmid to construct host-vector balance lethal system , and this system can be used to express foreign gene efficiently and to develop potential oral multivalent vaccines.<p /><p>Language: zh</p>",
language="zh",
issn="1000-484X",
doi="10.3969/j.issn.1000-484X.2014.08.017",
url="http://dx.doi.org/10.3969/j.issn.1000-484X.2014.08.017"
}