@article{ref1,
title="Construction and characterization of host-vector balanced lethal system of attenuated salmonella choleraesuisΔcrpΔcyaΔasdC78-1 (pYA3493)",
journal="Chinese Journal of Immunology",
year="2015",
author="Shang, Ke and Zhang, Junfeng and Cheng, Xiangchao and Zhang, Chunjie and Li, Yinju and Chen, Guihua and Yan, Yunfei and Zhai, Chongkai and Zhao, Zhanqin",
volume="",
number="12",
pages="358-363",
abstract="OBJECTIVE:In order to develop an oral live vaccine vector of swine that can stably carry exogenous genes.<br><br>METHODS:Mutant ΔcrpΔcyaΔasdC78-1 was constructed by the method of suicide plasmid pREasd-mediated bacteria homologous recombination on the basis of attenuated Salmonella choleraesuisΔcrpΔcyaC78-1.Complementary plasmid pYA3493 with asd was electrotransformed into the mutant,and thenΔcrpΔcyaΔasdC78-1(pYA3493) host-vector balanced lethal system was constructed.Its biological characteristics were analyzed further.<br><br>RESULTS:The results of PCR and sequencing showed thatΔcrpΔcyaΔasdC78-1(pYA3493) was constructed suc-cessfully.Biological characteristics showed that the serotype of ΔcrpΔcyaΔasdC78-1(pYA3493) was identical to ΔcyaΔasdC78-1 and vaccine strain C500 and it can stably carry theΔasd gene in vitro;its growth speed was a little slower than ΔcrpΔcyaC78-1 strain,but both of their growth speeds were significantly slower than vaccine strain C500;the biochemical characteristics of ΔcrpΔcyaΔasdC78-1 ( pYA3493 ) were basically the same with ΔcrpΔcyaC78-1 strain.Oral virulence test in mice showed that the virulence ofΔcrpΔcyaΔasdC78-1 ( pYA3493 ) was similar with ΔcrpΔcyaC78-1, but its median lethal dose is 412 times of vaccine strain C500.<br><br>CONCLUSION:These results demonstrated that attenuated Salmonella choleraesuisΔcrpΔcyaΔasdC78-1(pYA3493) strain had the potential to be used as an oral live vaccine vector for expressing foreign genes efficiently.<p /><p>Language: zh</p>",
language="zh",
issn="1000-484X",
doi="10.3969/j.issn.1000-484X.2015.03.015",
url="http://dx.doi.org/10.3969/j.issn.1000-484X.2015.03.015"
}