@article{ref1,
title="Cell-specific expression of the diphtheria toxin A-chain coding sequence induces cancer cell suicide",
journal="Chinese medical journal",
year="2002",
author="Rui, Hongbing and Chen, Yuanzhong",
volume="",
number="24",
pages="869-873",
abstract="&lt;p&gt;&lt;b&gt;OBJECTIVE&lt;/b&gt;To test whether the diphtheria toxin A (DT-A) chain coding sequence linked to murine immunoglobulin Kappa light chain (IgKappa) promoter and enhancer have selective cytocidal effects on IgKappa producing cells.&lt;/p&gt;&lt;p&gt;&lt;b&gt;METHODS&lt;/b&gt;The diphtheria toxin A gene or beta galactosidase (beta-gal) gene were linked to a murine IgKappa promoter and enhancer to construct pcDNA3IgKappaDTA or pcDNA3IgKappaLacZ plasmids. These plasmids were transfected into IgKappa producing or non-producing cells by the liposome coated DNA method. Expression of beta-gal activity and effects on cell growth of transfected cells were assessed.&lt;/p&gt;&lt;p&gt;&lt;b&gt;RESULTS&lt;/b&gt;The beta-gal gene, under the control of cytomegalovirus (CMV) promoter, can express in all cell lines. Expression of beta-gal under the control of the IgKappa promoter was detected only in the IgKappa producing cell line, CA46. Expression of beta-gal was greatly suppressed when cotransfected with pcDNA3IgKappaDTA in CA46 cells. Cell growth of CA46 cells transfected with pcDNA3IgKappaDTA plasmid was significantly inhibited compared with CA46 cells transfected with pcDNA3IgKappaLacZ.&lt;/p&gt;&lt;p&gt;&lt;b&gt;CONCLUSION&lt;/b&gt;Selective killing of IgKappa producing cells can be attained by introducing the diphtheria toxin A gene under the control of IgKappa promoter and enhancer.&lt;/p&gt;<p /><p>Language: en</p>",
language="en",
issn="0366-6999",
doi="",
url="http://dx.doi.org/"
}