@article{ref1,
title="All-trans retinoic acid sensitizes human medulloblastoma cells Med-3 to anti-Fas antibody mediated apoptosis",
journal="Chinese Journal of Immunology",
year="2000",
author="Guo, Li and Liu, Jia and Li, Junwei and Et, Al",
volume="",
number="12",
pages="-",
abstract="OBJECTIVE:It was aimed to highlight the pro apoptotic significance and immunotherapeutic value of the membrane type Fas(mFas)up regulated in all trans retinoic acid(RA)differentiated medulloblastoma cells Med 3.<br><br>METHODS:Med 3 cells were treated by 10 ?mol/L RA for 0,24,48 or 72 h,followed by the supplementation of anti Fas antibody in the final concentration of 25,50,100,200 or 400 ng/ml.The cell morphology ,growth and death pattern(s)were evaluated by multiple approaches.<br><br>RESULTS:RA could promote cell differentiation and mFas expression but not induce apoptosis.Anti Fas antibody alone had no biological effect on Med 3 cells but when combined with 10 ?mol/L RA,showed pro apoptotic activity in a dose related fashion.The best apoptotic induction could be achieved in the cell population pretreated by RA for 48 h.<br><br>CONCLUSION:The RA enhanced apoptotic susceptibility of Med 3 cells is closely associated with mFas up regulation.The chemical activation of Fas suicide system may be a novel regimen for medulloblastomas.<p /><p>Language: zh</p>",
language="zh",
issn="1000-484X",
doi="",
url="http://dx.doi.org/"
}