@article{ref1,
title="Adenovirus-mediated TK gene combined with α-IFN in treatment of nude mice transplanted with human renal clear-cell carcinoma",
journal="Academic journal of Second Military Medical University",
year="2010",
author="Zhao, Guo-Zhi",
volume="",
number="12",
pages="294-297",
abstract="OBJECTIVE: To investigate the inhibitory effect of adenovirus-mediated herpes simplex virus-thymidine kinase (TK) gene combined with α-IFN on renal clear-cell carcinoma. <br><br>METHODS: Adenovirus containing suicide gene TK, in combination with GCV or α-IFN, was used to treat human renal clear-cell carcinoma cell line 786-0, and the in vitro cytotoxic effects against 786-0 were evaluated using MTT method. The subcutaneous transplantation model of 786-0 cells was established with nude mice. Adenovirus containing TK gene was injected intratumorally and the GCV (50 mg/kg) was injected intraperitoneally; α-IFN (104 U/L) was injected intratumorally in combined therapy. The growth of tumors was observed after treatments. <br><br>RESULTS: The survival rate of 786-0 cells was (35.07 ± 1.43)% in the TK+GCV+α-IFN group, (68.57 ± 1.41)% in the TK+ GCV group and (68.65 ± 1.45)% in the α-IFN group (P=0.000). There was an obvious synergic effect between Ad-TK and α-IFN in inhibiting 786-0 cells. Ad-TK combined with GCV and α-IFN significantly suppressed the growth of 786-0 cells growth in nude mice model. <br><br>CONCLUSION: Adenovirus-mediated TK plus prodrug GCV combined with α-IFN has obvious therapeutic effect in treatment of human renal clear-cell carcinoma.<p /><p>Language: zh</p>",
language="zh",
issn="0258-879X",
doi="10.3724/SP.J.1008.2008.00294",
url="http://dx.doi.org/10.3724/SP.J.1008.2008.00294"
}