@article{ref1,
title="One-step, visual and sensitive detection of phorate in blood based on a DNA-AgNC aptasensor",
journal="New Journal of Chemistry",
year="2018",
author="Li, X. and Shi, J. and Chen, C. and Li, W. and Han, L. and Lan, L. and Guo, Y. and Chang, Y. and Cai, J. and Ding, Y.",
volume="42",
number="8",
pages="6293-6298",
abstract="The ingestion of organophosphate (Op) pesticides for attempting suicide has been a serious health issue recently. Phorate, a class of Op, has attracted attention in this regard and so the rapid diagnosis and detection of phorate is crucial for human health. In this study, a one-step and visual method for the rapid determination of phorate via an aptasensor based on DNA-templated silver nanoclusters (DNA-AgNCs) has been presented. The designed DNA-AgNC nanoprobe, containing a special structure with an intercalated aptamer, shows excellent stability. Introducing phorate to the system easily triggers the aggregation of DNA-AgNCs, producing a brown-to-colorless color change in the solution in only 6 minutes. Under the optimal conditions, the aptasensor can allow for the determination of phorate in the concentration range of 0-25 μg mL-1, using two linear equations: y = -2.308x + 0.996 (R2 = 0.977) for 0-0.125 μg mL-1 and y = -0.020x + 0.664 (R2 = 0.971) for 0.125-25 μg mL-1. The estimated detection limit of phorate was 0.012 ng mL-1. Moreover, the practicability of this proposed method was also validated by analyzing phorate-spiked human blood samples and the results were in agreement with those from gas chromatography/mass spectrometry (GC/MS) analysis. Therefore, this fabricated aptasensor is suitable for sensing phorate in complex biological samples, and shows high potential for clinical diagnosis or forensic toxicology analysis. © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique.<p /><p>Language: en</p>",
language="en",
issn="1144-0546",
doi="10.1039/c8nj00958a",
url="http://dx.doi.org/10.1039/c8nj00958a"
}