@article{ref1,
title="Transcriptome analysis reveals the peptide toxins diversity of Macrothele palpator venom",
journal="International journal of biological macromolecules",
year="2023",
author="Xiao, Xin and Luo, Xiaoqing and Huang, Cuiling and Feng, Xujun and Wu, Meijing and Lu, Minjuan and Kuang, Jiating and Peng, Siyi and Guo, Yingmei and Zhang, Zixuan and Hu, Zhaotun and Zhou, Xi and Chen, Minzhi and Liu, Zhonghua",
volume="ePub",
number="ePub",
pages="ePub-ePub",
abstract="Spider venom is a large pharmacological repertoire of different bioactive peptide toxins. However, obtaining crude venom from some spiders is challenging. Thus, studying individual toxins through venom purification is a daunting task. In this study, we constructed the cDNA library and transcriptomic sequencing from a pair of Macrothele palpator venom glands. Subsequently, 718 high-quality expressed sequence tags (ESTs) were identified, and grouped into three categories, including 449 toxin-like (62.53 %), 136 cellular component (18.94 %) and 133 non-matched (18.52 %) based on the gene function annotation. Additionally, 112 non-redundant toxin-like peptides were classified into 13 families (families A-M) based on their sequence homology and cysteine framework. Bioinformatics analysis revealed a high sequence similarity between families A-J and the toxins from Macrothele gigas in the NR database. In contrast, families K-M had a generally low sequence homology with known spider peptide toxins and unpredictable biological functions. Taken together, this study adds many new members to the spider toxin superfamily and provides a basis for identifying various potential biological tools in M. palpator venom.<p /> <p>Language: en</p>",
language="en",
issn="0141-8130",
doi="10.1016/j.ijbiomac.2023.126577",
url="http://dx.doi.org/10.1016/j.ijbiomac.2023.126577"
}