@article{ref1,
title="A rapid assay provides on-site quantification of tetrahydrocannabinol in oral fluid",
journal="Science translational medicine",
year="2021",
author="Yu, Hojeong and Lee, Hoyeon and Cheong, Jiyong and Woo, Sang Won and Oh, Juhyun and Oh, Hyun-Kyung and Lee, Jae-Hyun and Zheng, Hui and Castro, Cesar M. and Yoo, Yeong-Eun and Kim, Min-Gon and Cheon, Jinwoo and Weissleder, Ralph and Lee, Hakho",
volume="13",
number="616",
pages="eabe2352-eabe2352",
abstract="Tetrahydrocannabinol (THC), the primary psychoactive ingredient of cannabis, impairs cognitive and motor function in a concentration-dependent fashion. Drug testing is commonly performed for employment and law enforcement purposes; however, available tests produce low-sensitive binary results (lateral flow assays) or have long turnaround (gas chromatography-mass spectrometry). To enable on-site THC quantification in minutes, we developed a rapid assay for oral THC analysis called EPOCH, express probe for on-site cannabis inhalation. EPOCH features distinctive sensor design such as a radial membrane and transmission optics, all contained in a compact cartridge. This integrated approach permitted assay completion within 5 minutes with a detection limit of 0.17 ng/mL THC, which is below the regulatory guideline (1 ng/mL). As a proof of concept for field testing, we applied EPOCH to assess oral fluid samples from cannabis users (n = 43) and controls (n = 43). EPOCH detected oral THC in all specimens from cannabis smokers (median concentration, 478 ng/mL) and THC-infused food consumers. Longitudinal monitoring showed a fast drop in THC concentrations within the first 6 hours of cannabis smoking (half-life, 1.4 hours)., The express probe for on-site cannabis inhalation (EPOCH) system is able to quantitates tetrahydrocannabinol concentrations in oral fluid.<p /> <p>Language: en</p>",
language="en",
issn="1946-6234",
doi="10.1126/scitranslmed.abe2352",
url="http://dx.doi.org/10.1126/scitranslmed.abe2352"
}