@article{ref1,
title="Advantages of filtration method for sperm-DNA genotyping in sexual assault cases",
journal="Legal medicine (Elsevier)",
year="2021",
author="Nakagawa, Toshifumi and Doi, Masanori and Nishi, Kosuke and Sugahara, Takuya",
volume="54",
number="",
pages="e101988-e101988",
abstract="Differential extraction (DE) is a conventional method to isolate sperms from forensic semen samples (e.g. vaginal swab containing semen) for sperm-DNA genotyping. Subsequent to selective digestion of somatic cells in a mixture sample, sperms are collected and purified as a pellet by repetitive centrifugation based on the specific gravity of sperm heads. However, the centrifugation operation requires a technical proficiency and an extensive time to prevent a loss of sperms from the pellet as much as possible. Therefore, we devised a &quot;filtration method (FM)&quot;, in which a vacuum filtration operation based on the size of sperm heads is adapted, instead of DE, for isolation of sperms without any loss in principle. Sperms are collected and purified on a polycarbonate membrane filter. In this study, we have compared results of forensic assays by DE and FM for sperm-DNA genotyping from forensic semen samples. Consequently, FM had advantages of easy operation, timesaving, and high yield of sperms from semen samples compared with DE, although FM had a comparable ability to DE for a purification of sperms from mixture samples. Thus, we present that FM could simply lead to success of sperm-DNA genotyping and has a possibility to supersede DE as a gold-standard method.<p /> <p>Language: en</p>",
language="en",
issn="1344-6223",
doi="10.1016/j.legalmed.2021.101988",
url="http://dx.doi.org/10.1016/j.legalmed.2021.101988"
}