@article{ref1,
title="Short-term effect of gas explosion in real roadway environment on rats' brain neural behavior",
journal="Wei Sheng Yan Jiu",
year="2020",
author="Wu, Weidong and Yao, Sanqiao and Dong, Xinwen and Li, Haibin and Li, Juan and Sun, Lei and Weng, Xiaogang and Cao, Jia and Ren, Wenjie and Li, Runzhi",
volume="49",
number="6",
pages="889-894",
abstract="OBJECTIVE: To study the effect of gas explosion on brain nerve behavior of rats in real roadway environment. <br><br>METHODS: Before the gas explosion, the real gas explosion  roadway environment was simulated by using the roadway and explosion test system of  gas explosion test in a large coal mine in Chongqing Research Institute of China  Coal Science & Technology Group, and cage fixation and explosion parameter  setting were carried out. That was to use the equivalent of 9. 0% gas containing  mixed air and to install special cage in roadway gas detonation distance at point 40  m, 160 m and 240 m. The SPF grade healthy adult SD male rats anesthetized with  chloral hydrate were placed among them, and the rats were placed in a position that  could force the head. At the same time, the trunk part below the occipital foramen  and the mouth and face above the line of inner canthus were fully protected, and the  gas explosion experiment was carried out. A total of 40 rats were randomly divided  into four groups according to their body weight: control group, burn-blast combined  injuries group(40 m), proximal group(160 m) and distance group(240 m). Ten rats in  each group were placed in cages at different distance points under anesthesia except  the normal control group. The general physiological behavior of the rats was  observed 2 h and 7 d after the explosion, and the neurobehavioral indexes of the  rats were monitored by open field behavior experiment. Gross observation and  pathological examination of brain tissue were performed 7 days later. <br><br>RESULTS: The spirits of the rats in the 2 h exposure group after explosion were poor, and  improved slightly after 7 d. The degree of surface burn was the most serious in  group 40 m. The number of urination decreased while the number of feces  increased(P>0. 05). At the end of the experiment, it was found that cerebral  edema and hyperemia were obvious in rats. Compared with the normal control group,  the brain weight of rats in each exposure group increased, and the difference was  statistically significant(P<0. 05). Pathological observation showed that the  brain tissues of rats in each exposed group showed irregular and disordered  arrangement of nerve cells, interstitial edema, dense and deep staining of loose  nuclear chromatin, formation of dense mass and other characteristics of apoptotic  cells, as well as increased glia and aggregation of inflammatory cells. At 2 d and 7  h after the explosion, compared with the control group, the resting time of the  neurobehavioral indicators of rats at different distance points was significantly  prolonged(P<0. 01), while the number of standing times, movement time and  movement distance were significantly reduced, and the difference was statistically  significant(P<0. 01). <br><br>CONCLUSION: The gas explosion in real roadway environment  can cause certain damage to the brain tissue of rats, and has obvious influence on  its neural behavior.<p /> <p>Language: zh</p>",
language="zh",
issn="1000-8020",
doi="10.19813/j.cnki.weishengyanjiu.2020.06.003",
url="http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2020.06.003"
}