@article{ref1,
title="Development of a PCR high-resolution melt assay for artemisia absinthium (wormwood) and a triplex assay with two additional "unregulated legal high" species datura stramonium (jimson weed) and merremia tuberosa (Hawaiian woodrose)",
journal="Journal of forensic sciences",
year="2019",
author="Kiesel, Brianna D. and Elkins, Kelly M.",
volume="ePub",
number="ePub",
pages="ePub-ePub",
abstract="Artemisia absinthium (wormwood), a common ingredient in absinthe, contains the compound thujone, which is unregulated by the U.S. Drug Enforcement Agency. Thujone can cause an &quot;unregulated legal high&quot; in higher concentrations. The European Union limits thujone from Artemisia species to 35 mg/kg while the U.S. Food and Drug Administration requires less than 10 ppm to be &quot;thujone-free.&quot; However, individuals can smoke or ingest A. absinthium in different forms. This study developed a polymerase chain reaction (PCR) high-resolution melt (HRM) assay to detect and identify A. absinthium based on primer specificity, sensitivity, repeatability, and robustness. A triplex assay was performed with three &quot;unregulated legal high&quot; species: Datura stramonium, Merremia tuberosa, and A. absinthium; the PCR HRM assay detected and identified each plant at melt temperatures 77.42 ± 0.20°C, 83.88 ± 0.22°C, and 87.77 ± 0.15°C, respectively. The primer set developed distinguished A. absinthium from a variety of plant species and was successfully triplexed.<br><br>© 2019 American Academy of Forensic Sciences.<p /> <p>Language: en</p>",
language="en",
issn="0022-1198",
doi="10.1111/1556-4029.14093",
url="http://dx.doi.org/10.1111/1556-4029.14093"
}