@article{ref1,
title="Brain-enriched MicroRNA-184 is downregulated in older adults with major depressive disorder: a translational study",
journal="Journal of psychiatric research",
year="2019",
author="Mendes-Silva, Ana Paula and Fujimura, Patricia Tiemi and Silva, Jéssica Regina da Costa and Teixeira, Antonio Lucio and Vieira, Erica Marciano and Guedes, Pedro Henrique Gonçalves and Barroso, Lucélia Scarabeli Silva and Nicolau, Mariana de Souza and Ferreira, Jéssica Diniz Rodrigues and Bertola, Laiss and Nicolau, Eduardo de Souza and Tolentino-Araújo, Gesiane Thamire and Berlezzi, Camila Moreira Silva Ferreira and Rodrigues, Tamiris Sabrina and Borges, Luiza Diniz Ferreira and Gomes, Matheus de Souza and Amaral, Laurence Rodrigues do and Bonetti, Ana Maria and Ueira-Vieira, Carlos and Diniz, Breno Satler",
volume="111",
number="",
pages="110-120",
abstract="Changes in microRNAs (miRNAs) expression have been described in major depressive disorder in young and middle-aged adults. However, no study has evaluated miRNA expression in older adults with major depression (or late-life depression [LLD]). Our primary aim was to evaluate the expression of miRNAs in subjects with LLD. We first evaluated the miRNA expression using next-generation sequencing (NGS) and then we validated the miRNAs found in NGS in an independent sample of LLD patients, using RT-qPCR. Drosophila melanogaster model was used to evaluate the impact of changes in miRNA expression on behavior. NGS analysis showed that hsa-miR-184 (log<sub>2</sub>foldchange = -4.21, p = 1.2 × 10<sup>-03</sup>) and hsa-miR-1-3p (log<sub>2</sub>foldchange = -3.45, p = 1.3 × 10<sup>-02</sup>) were significantly downregulated in LLD compared to the control group. RT-qPCR validated the downregulation of hsa-miR-184 (p < 0.001), but not for the hsa-miR-1-3p. The knockout flies of the ortholog of hsa-miR-184 showed significantly reduced locomotor activity at 21-24 d.p.e (p = 0.04) and worse memory retention at 21-24 d.p.e (24h post-stimulus, p = 0.02) compared to control flies. Our results demonstrated that subjects with LLD have significant downregulation of hsa-miR-184. Moreover, the knockout of hsa-miR-184 in flies lead to depressive-like behaviors, being more pronounce in older flies.<br><br>Copyright © 2019 Elsevier Ltd. All rights reserved.<p /> <p>Language: en</p>",
language="en",
issn="0022-3956",
doi="10.1016/j.jpsychires.2019.01.019",
url="http://dx.doi.org/10.1016/j.jpsychires.2019.01.019"
}