@article{ref1,
title="Systemic inflammatory response and multiple organ dysfunctions following crush injury: a new experimental model in rabbits",
journal="Inflammation",
year="2018",
author="Xu, Peng and Wang, Fei and Zhou, Xian-Long and Li, Lei and Xiong, Dan and Yong, Yong-Quan and Zhao, Yan and Jiang, Wang-Xiang",
volume="41",
number="1",
pages="240-248",
abstract="In this study, we aim to develop a new, reproducible crush injury (CI) model in rabbits. Anesthetized rabbits were compressed on both hind limbs using a special instrument for 6 h followed by 3 h of reperfusion. Blood samples and injured muscles were collected for biochemical analysis and morphological evaluation. Survival observation lasted for 72 h. Bilateral compressions with 10 kg/kg body weight (BW), but not with 5 kg/kg BW, reduced serious systemic impairment. Bilateral compressions with 10 kg/kg BW resulted in severe lactic acidosis; increased serum K(+), creatine phosphokinase, aspartate transaminase, alanine transaminase, blood urea nitrogen, and creatinine levels; and a sharply decreased mean arterial blood pressure after compression release. Serious tissue edema and inflammation were observed in the damaged muscles. The mortality rates in compression groups were 20% (5 kg/kg BW) and 60% (10 kg/kg BW). There was a significant increase in plasma concentrations of TNF-α and IL-1β after compression. Plasma IL-1β levels returned to control levels at 6 h after compression release, whereas TNF-α peaked at 12 h following reperfusion. Furthermore, antiinflammatory cytokines, including IL-4 and IL-10, were also increased after compression, and these two cytokines peaked at 12 h after compression release. Our data suggested that bilateral compression with 10 kg/kg BW on rabbits' hind limbs is a reproducible CI model, and we also reported the CI-induced systemic inflammatory responses and changes of cytokines over time.<p /> <p>Language: en</p>",
language="en",
issn="0360-3997",
doi="10.1007/s10753-017-0683-5",
url="http://dx.doi.org/10.1007/s10753-017-0683-5"
}