@article{ref1,
title="Persian Gulf jellyfish (Cassiopea andromeda) venom fractions induce selective injury and cytochrome C release in mitochondria obtained from breast adenocarcinoma patients",
journal="Asian Pacific journal of cancer prevention",
year="2017",
author="Mirshamsi, Mohammad Reza and Omranipour, Ramesh and Vazirizadeh, Amir and Fakhri, Amir and Zangeneh, Fatemeh and Mohebbi, Gholam Hussain and Seyedian, Ramin and Pourahmad, Jalal",
volume="18",
number="1",
pages="277-286",
abstract="OBJECTIVE: This study was conducted to investigate whether fractions of jellyfish Cassiope andromeda venom, could selectively induce toxicity on mitochondria isolated from cancer tissue of patients with breast adenocarcinomas. <br><br>METHODS: Firstly, we extracted two fractions, (f1 and f2) from crude jellyfish venom by gel filtration on Sephadex G-200.Then different dilutions of these extracted fractions were applied to mitochondria isolated from human breast tumoral- and extra-tumoral tissues. Parameters of mitochondrial toxicity including generation of reactive oxygen species (ROS), mitochondrial membrane potential (MMP) collapse, swelling, cytochrome c release, activation of caspase3 and apoptosis were then assayed. <br><br>RESULT: Our results demonstrate that fraction 2 of Cassiopea andromeda crude venom significantly (P<0.05) decreased mitochondrial succinate dehydrogenase activity, increased mitochondrial ROS production, induced mitochondrial swelling, MMP collapse and cytochrome c release, activated caspase3 and induced apoptosis only in tumoral mitochondria, and not in mitochondria obtained from extra-tumoral tissue (P<0.05). <br><br>CONCLUSION: In conclusion this study suggested that fraction 2 of Cassiopea andromeda crude venom selectively induces ROS mediated cytotoxicity by directly targeting mitochondria isolated from human breast tumor tissue.<br><br>Creative Commons Attribution License<p /> <p>Language: en</p>",
language="en",
issn="2476-762X",
doi="10.22034/APJCP.2017.18.1.277",
url="http://dx.doi.org/10.22034/APJCP.2017.18.1.277"
}