@article{ref1,
title="Study on tetrodotoxin detection and species identification of pufferfish in retail roasted fish fillet by DNA barcoding in China",
journal="Food additives and contaminants: Part A, chemistry, analysis, control, exposure and risk assessment",
year="2015",
author="Nan, L. and Qing, S. and Hui, W. J. and Hui, H. C. and Rong, J. and Qin, L. F. and Tao, J.",
volume="32",
number="12",
pages="2148-2153",
abstract="The purpose of this study was to identify the pufferfish species and detect the tetrodotoxin (TTX) in roasted fish fillet samples which were collected in Beijing, Qingdao and Xiamen of China. Cytochrome c oxidase I (COI) gene was used as target gene for identification of the pufferfish species in samples. Enzyme-linked immunosorbent assay (ELISA) were used to screen the TTX levels in samples which had been detected as containing pufferfish by DNA barcode. 125 samples were identified by DNA barcodes, 32(26%) samples contained pufferfish composition and among them 26 (81%) were the highly toxic species named Lagocephalus lunaris. All 32 samples containing the pufferfish composition were positive for TTX with the levels ranging from 100 ng/g to 63,800 ng/g. Most of the 32 samples contained the highly toxic species Lagocephalus lunari. Based on the results, we suggest that the monitoring of roasted fish fillet should be strengthened and the processing procedures should be standardised to minimise TTX poisoning caused by pufferfish.<p /> <p>Language: en</p>",
language="en",
issn="1944-0049",
doi="10.1080/19440049.2015.1087056",
url="http://dx.doi.org/10.1080/19440049.2015.1087056"
}