@article{ref1,
title="Chlorpyrifos determined in human blood by UPLC-MS/MS and its application in poisoning cases",
journal="Fa Yi Xue Za Zhi",
year="2015",
author="Qiao, Zheng and Yan, Hui and Zhuo, Xian-yi and Shen, Bao-hua",
volume="31",
number="2",
pages="112-116",
abstract="OBJECTIVE: To determine the chlorpyrifos in human blood by liquid chromatography-tandem mass spectrometry and to validate its application in poisoning cases. <br><br>METHODS: The samples were extracted by a simple one-step protein precipitation procedure. Chromatography was performed on a Capcell Pack C18 MGII column (250 mm x 2.0 mm, 5 μm) using an isocratic elution of solvent A (0.1% formic acid-water with 2 mmol/L ammonium acetate) and solvent B (methanol with 2 mmol/L ammonium acetate) at 5:95 V:V). <br><br>RESULTS: The linear ranged from 5 to 500 ng/mL (r = 0.998 7). The limit of detection (LOD) and the lower limit of quantification (LLOQ) were 2 ng/mL and 4 ng/mL, respectively. For this method, the precision and accuracy of intra-day and inter-day were < 10% and 97.44%-101.10%, respectively. The results in stability test of long-term frozen were satisfied. The matrix effect, recovery and process efficiency were 64.97%-86.81%, 76.70%-85.52%, and 55.57%-66.58%, respectively. <br><br>CONCLUSION: This method can provide a rapid approach to chlorpyrifos extraction and determination in toxicological analysis of forensic and clinical treatment.<p /> <p>Language: en</p>",
language="en",
issn="1004-5619",
doi="",
url="http://dx.doi.org/"
}