@article{ref1,
title="Point-of-care diagnostics for ricin exposure",
journal="Lab on a chip",
year="2015",
author="Diakite, Mohamed Lemine Youba and Rollin, Jérôme and Jary, Dorothée and Berthier, Jean and Mourton-Gilles, Chantal and Sauvaire, Didier and Philippe, Cathy and Delapierre, Guillaume and Gidrol, Xavier",
volume="15",
number="10",
pages="2308-2317",
abstract="A long-sought milestone in the defense against bioterrorism is the development of rapid, simple, and near-patient assays for diagnostic and theranostic purposes. Here, we present a powerful test based on a host response to a biological weapon agent, namely the ricin toxin. A signature for exposure to ricin was extracted and characterized in mice and then integrated into a plastic microfluidic cartridge. This enabled early diagnosis of exposure to ricin in mice using a drop of whole blood in less than 1 h and 30 min. The cartridge stores the reagents and implements all of the steps of the analysis, including mRNA extraction from a drop of blood, followed by tens of parallel RT-qPCR reactions. The simple and low-cost microfluidic cartridge developed here may find other applications in point-of-care diagnostics.<p /> <p>Language: en</p>",
language="en",
issn="1473-0197",
doi="10.1039/c5lc00178a",
url="http://dx.doi.org/10.1039/c5lc00178a"
}