@article{ref1,
title="A Molecular Method to Correlate Bloodstains with Wound Site for Crime Scene Reconstruction",
journal="Journal of forensic sciences",
year="2014",
author="Johnson, Donald J. and Andersen, Cheryl and Scriven, Katherine A. and Klein, Amberly N. and Choi, Mo Re and Carroll, Cindy and de Leon, Ray D.",
volume="59",
number="3",
pages="735-742",
abstract="Bloodstain pattern analysis to determine the wound-of-origin of bloodstains is problematic with nonspecific patterns. In this proof-of-concept study, the authors examined a molecular approach to correlate bloodstains with injuries using the rat as a model. Specifically, investigations were conducted on the rat brain marker, rno-miR-124-3p, with the QIAGEN miScript System and real-time PCR analysis. Rno-miR-124-3p was detected in brain homogenates diluted 100,000 times; in 3-week-old, room temperature stored, simulated brain-blood stains; and in bloodstains from head gunshot wounds collected with swabs and subsequently frozen for 9-18 months; however, rno-miR-124-3p was not detected in whole blood. Proof-of-principle was demonstrated by the ability to distinguish bloodstains from a gunshot wound to the head versus bloodstains from a gunshot wound to the chest, by the testing of otherwise identical bloodstains from the two patterns for the presence of the marker. The results suggest a viable approach to a longstanding problem in casework.<p /><p>Language: en</p>",
language="en",
issn="0022-1198",
doi="10.1111/1556-4029.12377",
url="http://dx.doi.org/10.1111/1556-4029.12377"
}