@article{ref1,
title="A PCR marker Linked to a THCA synthase Polymorphism is a Reliable Tool to Discriminate Potentially THC-Rich Plants of Cannabis sativa L",
journal="Journal of forensic sciences",
year="2014",
author="Staginnus, Christina and Zörntlein, Siegfried and de Meijer, Etienne",
volume="59",
number="4",
pages="919-926",
abstract="Neither absolute THC content nor morphology allows the unequivocal discrimination of fiber cultivars and drug strains of Cannabis sativa L. unequivocally. However, the CBD/THC ratio remains constant throughout the plant's life cycle, is independent of environmental factors, and considered to be controlled by a single locus (B) with two codominant alleles (BT and BD ). The homozygous BT /BT genotype underlies the THC-predominant phenotype, BD /BD is CBD predominant, and an intermediate phenotype is induced by the heterozygous state (BT /BD ). Using PCR-based markers in two segregating populations, we proved that the THCA synthase gene represents the postulated B locus and that specific sequence polymorphisms are absolutely linked either to the THC-predominant or the THC-intermediate chemotype. The absolute linkage provides an excellent reliability of the marker signal in forensic casework. For validation, the species-specific marker system was applied to a large number of casework samples and fiber hemp cultivars.<p /><p>Language: en</p>",
language="en",
issn="0022-1198",
doi="10.1111/1556-4029.12448",
url="http://dx.doi.org/10.1111/1556-4029.12448"
}